Use of Molecular Biological Methods to Identify Plant Parasitic Nematodes Associated with Sugarcane in South Africa
نویسندگان
چکیده
Plant parasitic nematode genera commonly found associated with sugarcane were collected from trial sites in various regions of the sugar industry. Nucleic acids were extracted and the rDNA internal transcribed spacer 1 (ITS1) region amplified. Identification of the important genera was possible by size differentiation of the amplification products. Sequencing of the ITS1 region was used to confirm the identity of the isolates, by comparison with sequences in GenBank.
منابع مشابه
The Effect of Green Manure Crops on Plant Parasitic Nematodes in the South African Sugar Industry
Plant parasitic nematodes, Meloidogyne javanica in particular, cause significant yield losses in sugarcane crops in South Africa. The chemicals at present available for nematode control are expensive and potentially detrimental to the environment. It has been reported that various types of crops reduce the numbers of plant parasitic nematodes. Seven green manure crops (sunn hemp, marigold, oats...
متن کاملOrganic Amendments Enhance Biological Suppression of Plant-parasitic Nematodes in Sugarcane Soils
Previous research has shown that population densities of plant-parasitic nematodes are reduced when a legume crop is grown in rotation with sugarcane. However, this effect is only temporary, as nematodes usually return to high densities within 12 months of planting sugarcane. This rapid resurgence suggests that natural enemies that normally keep plant-parasitic nematodes under control in natura...
متن کاملReport of five species of plant parasitic nematodes associated with hawthorn forest trees in western Iran
Z, Bazgir E, Naghavi A (2020) Report of five species of plant parasitic nematodes associated with hawthorn forest trees in western Iran. Plant Pathology Science 9(1):16-30. DOI: 10.2982/PPS.9.1.16 Introduction: The Hoplolaimidae family is one of the largest and economically most important families of the order/ infraorder Tylenchida / Tylenchomorpha. The members of this family spread all over ...
متن کاملFactors associated with the suppressiveness of sugarcane soils to plant-parasitic nematodes.
Observations in three Australian sugarcane fields suggested that the soil just under the trash blanket (the covering of crop residue that remains on the soil surface after crops are harvested) was suppressive to plant-parasitic nematodes. Roots were concentrated in this upper layer of soil but plant-parasitic nematode populations were relatively low and roots showed few signs of nematode damage...
متن کاملPossible application of a nematophagous fungus as a biological control agent of parasitic nematodes on commercial sheep farms in South Africa.
Biological control of parasitic nematodes of livestock is currently under development and represents another tool that may be integrated into helminth parasite control strategies. This paper presents a brief introduction to commercial sheep farming in South Africa and currently available nematode parasite control methods. These include the FAMACHA clinical assay, strategies of pasture managemen...
متن کامل